• Potomac Photonics
  • January 26, 2021

Microfluidic Concentration Gradient Generators – Potomac Photonics

Microfluidic Concentration Gradient Generators – Potomac Photonics

Joe La Fiandra

University of Maryland College Park (UMCP)

Major: Bioengineering

Minor: Global Engineering Leadership

 

Microfluidic Concentration Gradient Generators

Many biochemical processes in biological organisms are controlled by concentration gradients. From cell migration to drug proliferation, concentration gradients are integral to the transportation of a vast array of substances throughout the body. Until recently, concentration gradient generation (CGG) platforms were simply too large to control molecular diffusion of reagents, resulting in premature comingling of fluids (source). Because of their prohibitive size, previous CGGs prevented identical replication of volumes and flow rates found in vivo, thereby tainting experimental data relevant to microscopic biochemical gradients. Therefore, modeling gradients on a microscopic scale is extremely valuable to understanding these complex interactions and the mechanics that enable them. 

With innovative rapid-prototyping technologies, Potomac Photonics has developed a process for manufacturing microfluidic concentration gradient generators (µ-CGGs). Constructed on a polydimethylsiloxane (PDMS) substrate bonded to glass, this device provides a low cost, easily executable method of investigating gradient-driven mechanisms. This platform leverages laminar flow, a fluid dynamic phenomena, and fluid dynamic geometries required to control the movement of reagents. In the µ-CGG, serpentine features allow for controlled diffusion of the reagents, forming mixtures of varying concentrations throughout the device. Designed with a tree-shaped network, the µ-CGG replicates the experiment 39 times at varying concentrations all on the same chip to create the gradient seen in the image. Using flow rates and volumes on a micro order of magnitude, µ-CGGs mimic a nearly-identical environment to that of a biochemical concentration gradient naturally found in biological organisms. 

While this µ-CGG design utilizes only 39 nodes in its flow network, the versatility, design potential, and low-cost of microfluidic device fabrication allow for a plethora of adjustments to complicate or simplify a µ-CGG. The sheer size of a microfluidic device is enough to enable multi-layer µ-CGGs to contain multiple gradients that flow into each other, or different flow levels resulting in dozens of combinations of several reagents. The ease of manufacturing µ-CGGs is particularly valuable. Primarily made out of elastomers and thermoplastics, µ-CGGs can be manufactured in substrates with specific material properties that enable complex chemical reactions to occur for relatively low cost. Leveraging all of these attributes, engineers can design µ-CGGs to their unique specifications on a single chip, decreasing time, materials, and personnel necessary for experimental set up and increasing volume of accurate data on a concise device.



Other Posts

Precision Laser Drilling of Glass Slides and Coverslips for Microfluidics and Biotech Applications

In the field of microfluidics, where precision is key to innovation and application success, our expertise at Potomac Photonics sets a standard. We specialize in the art of laser drilling precise small holes in glass slides and coverslips, a critical capability for the fabrication of microfluidic devices. These devices play a pivotal role in various…

  • Potomac Photonics
  • February 5, 2024

Machine Learning: Applying AI to Manufacturing at Potomac Photonics

The world today seems consumed with fears about Artificial Intelligence, otherwise known as AI. Writers, journalists, ad copywriters, teachers, and artists are fearful their jobs will be lost to computers but in manufacturing we have been applying a subset of AI to manufacturing for decades with positive results. Like other forms of AI, Machine Learning…

  • Potomac Photonics
  • November 12, 2023

Microfluidics: The Evolution from PDMS to Thermoplastics for Enhanced Scalability and Cost-Effectiveness

In the ever-evolving realm of microfluidics, the pivot from traditional materials to advanced alternatives is becoming increasingly evident. These “labs-on-a-chip” devices, pivotal in biological research, diagnostics, and drug delivery, owe their efficacy to the careful selection of materials and manufacturing methodologies. Historically, Polydimethylsiloxane (PDMS) has dominated the microfluidic landscape. Its biocompatibility, flexibility, and innate transparency…

  • Mike Adelstein
  • August 11, 2023